Hi

If you use lysozyme, it binds with bentonite, and thus is inactivated by
it. I find there are fewer filtration problems if I use bentonite a
10-14 days after adding the lysozyme to my wines. Since lysozyme is not
removed by filtration, it is a good idea to deactivate it prior to
filtration.

The specifics of lysozyme use are in on the Scott site that Pp provided
above.

Regards

Jerry

wrote in message
news:1109856186.353025@s0...
William Frazier wrote:
Lee - If you have malic and lactic acid spots on your chromatography
paper
you have a ML fermentation in process. In some of my wines that have
a MLF
underway it's very difficult to see bubbles at first. The wines have
just
been cloudy like you mention. As the temperature warms up and/or
after the
bacteria have really increased in number ML fermentation is more
apparent
with lots of bubbling.

I'm open to suggestions. Lysozyme addition? Coarse filtration
followed by
sterile filtration? Re-chilling? Gelatin? All of the above?

I read here that sulfite will stop or prevent MLF. This may be so if
the
sulfite is added before a MLF gets underway or if enough SO2 is
added.
However, I've had MLF take place in some of my wines that I believed
were
well protected with sulfite. Since I add ML culture to my red wines
the
organisms are just floating around in my cellar and it's real hard to
prevent MLF in my white wines.

I had good success stopping MLF by adding Lysozyme at 200 to 500 ppm
(at
least 0.75 grams per gallon) in cloudy wines that show malic and
lactic acid
spots like you describe. The wines I've treated have become crystal
clear
in a couple of days.

Once you get the MLF stopped and the wine clears your 0.2 micron
Duofine
filter will render the wine sterile. But sterile operation is next
to
impossible in typical basement operations. Everything downstream
from the
filter has to be sterile...that's the big problem. But, proper SO2
levels,
K sorbate at 200 ppm and sterile filtration should be enough.

Check out PIWC, Scottlab or The Winelab for some info on Lysozyme.
Good
luck.

Bill Frazier
Olathe, Kansas USA


Thanks, Bill. I got some lysozyme from PI. May give that a shot...

--Lee